Human BSEP Vesicle Kit
HEK293-derived inside-out membrane vesicles for BSEP inhibition screening and drug-induced liver injury (DILI) risk assessment, in line with FDA and EMA drug-drug interaction testing recommendations.
Regulatory Relevance
BSEP inhibition testing is recommended by FDA and EMA guidance for the assessment of drug-induced cholestasis and liver injury risk.
Inside-Out Vesicles
HEK293-derived membrane vesicles with confirmed BSEP expression and inside-out orientation for reliable, reproducible transport data.
100 Reactions per Kit
Each kit is sized for 100 reactions based on a 96-well plate format, suitable for IC50 determination and single-concentration screening.
Dry Ice Shipment
Shipped frozen at -80°C on dry ice to preserve vesicle integrity and transport activity upon arrival.
Why BSEP testing matters in DMPK programs
The Bile Salt Export Pump (BSEP, ABCB11) is the primary hepatic transporter responsible for the efflux of bile acids from hepatocytes into bile. Inhibition of BSEP is one of the most established mechanisms underlying drug-induced cholestasis and is a recognised contributor to drug-induced liver injury (DILI).
The vesicle-based assay uses taurocholic acid as a probe substrate to quantify BSEP-mediated transport into inside-out membrane vesicles, in the presence and absence of test compounds, allowing determination of inhibition potency and IC50 values.
Because dual inhibition of BSEP together with MRP2, MRP3 or MRP4 can impair the compensatory bile acid efflux pathways that partially protect hepatocytes, a complete transporter panel alongside BSEP often provides a more complete DILI risk profile than BSEP testing in isolation.
Typical applications
- Drug-induced liver injury (DILI) risk assessment
- Drug-drug interaction (DDI) studies per FDA / EMA guidance
- Hepatotoxicity screening in lead optimisation
- IC50 determination for BSEP inhibitors
- Regulatory transporter panels (BSEP, BCRP, P-gp, MRP1-5, MRP8)
- Academic and pharmaceutical DMPK research
Product information
- Product type: Human BSEP Vesicle Kit
- Source: HEK293-derived inside-out membrane vesicles
- Kit size: 100 reactions (96-well plate format)
- Substrate: Taurocholic acid
- Storage: -80°C
- Shipment: Dry ice
Available on request
- Certificate of Analysis (CoA)
- Technical data sheet
- Assay protocol support
- Full ABC transporter panel quote
- Bulk order options
Need a complete ABC transporter panel?
Contact our team for BSEP, BCRP, P-gp and MRP kit pricing, technical documentation and assay support for your DMPK study.
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