3D Single-Use Bioprocessing Bags — 50 L–1,000+ L | MPX | GMP | SeamlessBio
3D Single-Use Bioprocessing Bags — 50 L to 1,000+ L | MPX | GMP-Compatible
3D bioprocessing bags are cube-shaped single-use containers designed for large-volume bioprocessing in standard tote containers. Unlike flat 2D bags, 3D bags are engineered to fill and hold their cubic shape when placed inside stainless steel or HDPE bioprocessing containers — eliminating the need for cleaning, cleaning validation, and the risk of cross-contamination between batches.
SeamlessBio 3D bags are engineered as 1:1 drop-in replacements for Sartorius Flexel® and Thermo Fisher Hyperforma® systems. Pre-assembled with high-quality MPX (Body/Stem) connectors for secure, quick, and aseptic connections. All bags are gamma sterilised and shipped with full GMP documentation.
Compatible with: Sartorius Flexel® cube containers and totes | Thermo Fisher Hyperforma® systems | All standard stainless steel and HDPE cube containers. Fits existing holders, load cells, and mixing systems without process changes.
Key Applications
| Application | Details | Typical Volume |
|---|
| Large-scale media preparation | Prepare cell culture media in 50–1,000 L batches. Compatible with overhead mixers and pad mixers on bioprocessing containers. | 50–500 L |
| Buffer preparation & storage | Prepare, equilibrate and store large volumes of PBS, column buffers, process buffers — eliminate tank cleaning. | 100–1,000 L |
| Bulk intermediate storage | Store harvest material, concentrated protein, or intermediate product before downstream processing — maintain sterility without stainless steel. | 50–1,000 L |
| mAb manufacturing (upstream) | Media prep and feed preparation for large bioreactor runs. Integrate with CHO/NS0 perfusion and fed-batch processes. | 100–1,000 L |
| Vaccine production | Large-volume media and buffer preparation for viral vaccine manufacturing. Single-use eliminates cross-contamination between vaccine strains. | 100–500 L |
| Downstream processing | Hold tanks for chromatography loading, TFF feed, formulation buffer — replace stainless steel hold tanks in downstream. | 50–1,000 L |
Specifications
| Parameter | Specification |
|---|
| Volume range | 50 L, 100 L, 200 L, 500 L, 1,000 L | Larger volumes on request |
| Film material | USP Class VI certified multilayer film — E&L tested |
| Sterilisation | Gamma irradiated — ≥25 kGy, validated per ISO 11137 |
| Industry compatibility | 1:1 drop-in for Sartorius Flexel® and Thermo Fisher Hyperforma® tote systems |
| Standard connectors | MPX (Body/Stem) pre-assembled — secure, quick, aseptic connection |
| Port configurations | Standard: inlet, outlet, vent, sampling, drain — fully customisable |
| Manufacturing | ISO 13485 certified facility, Germany |
| Documentation | CoA, CoO, gamma sterility certificate, E&L data on request |
| GMP compliance | cGMP compatible — full documentation package available |
| Lead time | Standard: 2–4 weeks | Custom manifolds: 6–12 weeks |
| Minimum order | 10 bags (standard) | Custom from 100 bags |
Customisation Options
| Option | Details |
|---|
| Custom manifolds & tubing sets | Fully customised manifold designs — add sterile connectors, specify tubing lengths, add ports |
| Connector types | MPX standard, Luer lock, TC, AseptiQuik, custom sterile connectors |
| Film upgrades | Enhanced E&L profile, cryogenic-rated film for freeze-thaw applications |
| GMP documentation | Extended CoA, validation data, regulatory support files — on request |
| Custom printing | Company logo, batch codes, product codes on bag or outer packaging |
Single-Use Bioprocessing Portfolio
Order 3D Bags in the shop
Request a bulk quote or custom design
Custom manifolds, bulk pricing, GMP documentation: info@seamlessbio.de | +49 851 37932226
Flexel® and Flexboy® are registered trademarks of Sartorius Stedim Biotech. Hyperforma® is a trademark of Thermo Fisher Scientific. Mobius® is a trademark of Merck KGaA. SeamlessBio is an independent provider of compatible solutions and is not affiliated with or endorsed by these companies.
Applications Using This Product
| Application | Which Bag |
|---|
| CAR-T Cell Manufacturing → | Rocker Bag 0.5–50 L for ex vivo T cell expansion. 2D Bags for media and formulation buffer. |
| AAV & Viral Vector Production → | Rocker Bag for HEK293 suspension production. 2D Bags for virus harvest and buffer storage. |
| Vaccine Production → | Rocker Bag for Vero/MDCK seed train. 2D Bags for virus harvest collection. 3D Bags for large-volume media preparation. |
