Chicken Serum – USA/EU Origin
Species-Specific Supplement for Avian Cell Culture | Perfect for Immunoassays & Blocking | Sterile Filtered
What is Chicken Serum (Avian Serum)?
Chicken Serum (also called Avian Serum or Gallus gallus Serum) is a specialized biological reagent derived from healthy chickens in the USA or EU. Unlike mammalian sera such as FBS or horse serum, chicken serum is specifically optimized for avian cell culture systems and provides species-specific growth factors and proteins essential for chicken cell lines.
Our Chicken Serum is sterile filtered (0.2 µm) and sourced from USDA-inspected facilities (USA) or EU-certified abattoirs (Europe), making it ideal for:
- Avian cell culture – Chicken keratinocytes, muscle satellite cells, embryonic stem cells (cESC)
- Immunoassay blocking buffer – ELISA, Western blot, IHC, IF with chicken antibodies
- Antibody diluent for avian immunological studies
- Parasitology research – Leishmania cultivation as FBS alternative
- Vaccine development – Avian virus propagation and CEF culture support
💡 Species-Specific Advantage: Chicken serum contains the natural growth factors, hormones, and attachment proteins that avian cells require for optimal proliferation and differentiation. For chicken-derived cell lines, it often outperforms FBS in supporting cell health and maintaining proper morphology.
Chicken Serum vs. FBS: Which Do You Need?
| Feature | Chicken Serum (This Product) | Fetal Bovine Serum (FBS) |
|---|---|---|
| Source | Healthy adult chickens (USA/EU) | Bovine fetal blood |
| Best For | ✓ Avian cell lines, chicken research | General mammalian cell culture |
| Species Specificity | ✓ Optimized for chicken cells | Broad spectrum mammalian |
| Growth Factors | ✓ Avian-specific factors | Mammalian growth factors |
| Immunological Studies | ✓ No cross-reactivity with avian antibodies | May cross-react in bovine studies |
| Parasitology | ✓ Cost-effective for Leishmania | Standard but more expensive |
| Ethical Concerns | ✓ Adult animal source | Fetal source considerations |
| Price | Moderate – specialized product | Higher cost, market-dependent |
🎯 Quick Guide:
• Working with chicken cells? → Chicken Serum is your best choice
• Need blocking for chicken antibodies? → Chicken Serum prevents cross-reactivity
• Culturing Leishmania on budget? → Chicken Serum works as FBS alternative
• CEF cultures for vaccine production? → Chicken Serum supports optimal growth
• General mammalian cell culture? → Stick with FBS
Product Features & Quality Standards
🇺🇸🇪🇺 USA/EU Origin
Sourced from healthy chickens in USDA-inspected facilities or EU-certified abattoirs under veterinary supervision.
🔬 Sterile Filtered
0.2 µm filtration removes bacteria and particulates while preserving proteins and growth factors.
🐓 Species-Specific
Contains natural avian growth factors and proteins optimal for chicken cell culture systems.
🧪 Low Endotoxin
≤50 EU/mL ensures minimal inflammatory response in sensitive cell culture applications.
✅ Quality Tested
Every batch tested for sterility, mycoplasma, hemoglobin, pH, osmolality, and total protein.
📜 ISO Certified
Manufactured under ISO 9001 quality management with complete documentation (COA, MSDS).
Technical Specifications
Origin: United States (USDA-inspected) or European Union (EU-certified)
Source: Healthy adult chickens (Gallus gallus)
Filtration: 0.2 µm sterile filtered
Total Protein: 4.0-7.0 g/dL (batch-specific)
pH: 7.0-8.5
Osmolality: 260-340 mOsm/kg
Hemoglobin: ≤60 mg/dL
Endotoxin: ≤50 EU/mL
Quality Testing
| Test | Method | Specification |
|---|---|---|
| Sterility | USP <71> sterility test | Negative (aerobic, anaerobic, fungi) |
| Mycoplasma | PCR & culture | Not detected |
| Hemoglobin | Spectrophotometry | ≤60 mg/dL |
| Total Protein | Bradford assay | 4.0-7.0 g/dL |
| Endotoxin | LAL assay | ≤50 EU/mL |
| pH | pH meter | 7.0-8.5 |
| Osmolality | Osmometer | 260-340 mOsm/kg |
Documentation provided: Certificate of Analysis (COA), Certificate of Origin (COO), and Material Safety Data Sheet (MSDS) for every batch.
Primary Applications of Chicken Serum
🧬 Avian Cell Culture
Most popular use: Chicken serum is the optimal supplement for culturing chicken-derived cell lines, providing species-specific growth factors that mammalian sera cannot offer.
- Serial cultivation of chicken keratinocytes
- Primary chicken muscle satellite cell culture (proliferation at 10-15%, differentiation at 3%)
- Chicken embryonic stem cell (cESC) culture systems
- Primordial germ cell (PGC) culture and expansion
- Chicken embryo fibroblast (CEF) cultures for vaccine production
🔬 Immunological Research
Ideal blocking agent and antibody diluent when working with chicken-derived antibodies or studying avian immune responses.
- Blocking buffer in ELISA, Western blot, IHC, IF (prevents cross-reactivity with chicken antibodies)
- Antibody diluent for immunological assays
- Development of avian diagnostic assays
- Avian immunology research and vaccine studies
🦠 Parasitology & Virology
Cost-effective alternative to FBS for certain parasites and essential for avian virus work.
- Cultivation of Leishmania parasites (effective, economical FBS alternative)
- Avian virus propagation and vaccine development
- Trematode culture and developmental studies
- Support for chicken embryo-based vaccine production
🧪 Bioprocessing & Diagnostics
- Vaccine manufacturing using avian cell substrates
- Recombinant protein production in avian expression systems
- Cell-based assay development for poultry industry
- Avian disease diagnostic test development
💡 Recommended Concentrations:
• Chicken keratinocytes: 5-10% (v/v) in DMEM or Medium 199
• Muscle satellite cells (proliferation): 15% or 10% chicken serum + 5% horse serum
• Muscle satellite cells (differentiation): 3% (v/v)
• Embryonic stem cells (cESC): 10-15% (can combine with KSR)
• Immunoassay blocking: 3-10% (v/v) in PBS/TBS
Available Treatment Options
Standard (Sterile Filtered) – Recommended
Our default 0.2 µm sterile filtered chicken serum preserves all proteins, immunoglobulins, and growth factors. This is the preferred option for:
- Avian cell culture applications
- Immunoassay blocking and antibody dilution
- Parasitology and virology research
Heat-Inactivated
Heat inactivation (56°C, 30 min) inactivates complement proteins. Choose this when:
- Working with complement-sensitive cells
- Conducting antibody neutralization assays
- Propagating viruses where complement could interfere
- Running immunological assays with potential complement interference
⚠️ Note: Heat inactivation may slightly reduce heat-sensitive proteins. For most cell culture applications, standard (non-heat-inactivated) is preferred. Contact us if unsure which option is best for your application.
Gamma-Irradiated
Gamma irradiation (25-45 kGy) provides additional viral safety without heat exposure. Ideal for:
- GMP-grade manufacturing processes
- Applications requiring maximum viral safety
- Heat-sensitive protocols
Custom Treatments Available on Request
- Charcoal-stripped (hormone removal)
- Delipidized (reduced lipid content)
- Custom filtration specifications
- Specific volume aliquoting
Packaging Sizes & Volume Discounts
Available Sizes
| Volume | Cat. No. | Treatment | Typical Use |
|---|---|---|---|
| 100 mL | CS-USA-100-HI / CS-EU-100-HI | Heat-Inactivated | Trial size for testing |
| 500 mL | CS-USA-500-HI / CS-EU-500-HI | Heat-Inactivated | ✓ Most popular for regular use |
| 100 mL | CS-USA-100-GI / CS-EU-100-GI | Gamma-Irradiated | GMP applications |
| 500 mL | CS-USA-500-GI / CS-EU-500-GI | Gamma-Irradiated | Large-scale GMP work |
| Bulk Volumes | Custom | As specified | Contact for quote |
Volume Discounts
We offer attractive tiered pricing for larger orders:
- 5-10 L: 10% discount
- 10-25 L: 15% discount
- 25-50 L: 20% discount
- 50+ L: Custom pricing – contact us for quote
Storage & Handling
Long-term storage: -20°C to -70°C (up to 48 months shelf life)
After thawing: 2-8°C, use within 4 weeks
Thawing: Overnight at 2-8°C (recommended) or 37°C water bath with gentle mixing
Important: Avoid repeated freeze-thaw cycles (maximum 3 cycles)
🚚 Fast Shipping: Orders ship within 1-2 business days on dry ice with temperature monitoring. Express delivery available across EU and international shipping to USA, Canada, Australia, and Asia.
Batch Testing & Reservation Service
Ensure consistent performance for your critical applications with our comprehensive batch testing and reservation program:
How It Works:
- Request Free Test Sample – Contact us for a 50-100 mL test volume with complete batch documentation
- Batch Reservation – We reserve your selected batch quantity (up to 50 liters) with free cold storage
- Testing Period – Evaluate the batch in your specific application for up to 6 weeks (extendable by agreement)
- Receive Quotation – Get binding quote for the tested batch with no payment required during testing phase
- Scheduled Deliveries – Your batch is stored in our temperature-controlled facility for call-off as needed
Benefits: No upfront payment required • Free cold storage • Flexible delivery scheduling • Guaranteed batch consistency • Complete traceability • Long-term supply security for your projects
Frequently Asked Questions
Can chicken serum replace FBS in my cell culture?
Chicken serum is species-specific and optimal for avian cell lines. For chicken-derived cells (keratinocytes, satellite cells, cESC, PGCs), it often outperforms FBS in supporting proper cell morphology and function. For mammalian cells, it can work in certain applications (e.g., Leishmania culture) but requires validation. Always perform side-by-side testing before completely switching.
What's the difference between USA and EU origin chicken serum?
Both origins meet the same quality standards with 0.2 µm sterile filtration and comprehensive testing. USA origin comes from USDA-inspected facilities, while EU origin comes from EU-certified abattoirs. Performance is equivalent – choose based on your regulatory requirements or regional preference. Documentation includes Certificate of Origin specifying exact source.
How should I supplement my culture medium with chicken serum?
Typical concentrations: 5-10% for chicken keratinocytes, 15% (or 10% chicken + 5% horse serum) for muscle satellite cell proliferation, 3% for differentiation, 10-15% for embryonic stem cells. For immunoassay blocking, use 3-10% in PBS/TBS. Always optimize for your specific cell line and experimental conditions.
Do I need heat-inactivated or standard chicken serum?
For most applications (cell culture, blocking, antibody dilution), use standard (non-heat-inactivated). Heat-inactivated is only necessary when complement activity could interfere, such as in complement-sensitive cell culture, antibody neutralization assays, or certain virus work. If unsure, start with standard – you can always request heat-inactivated later.
Is chicken serum suitable for vaccine production?
Yes. Chicken serum is used in vaccine manufacturing for avian virus propagation and CEF (chicken embryo fibroblast) culture systems. For GMP-grade vaccine production, we recommend gamma-irradiated chicken serum with complete regulatory documentation. Contact our team to discuss your specific GMP requirements.
How long can I store chicken serum after opening?
After thawing, store at 2-8°C and use within 4 weeks. For longer storage, divide into smaller aliquots before freezing to avoid repeated freeze-thaw cycles (maximum 3 cycles recommended). At -20°C to -70°C, unopened vials maintain quality for up to 48 months from manufacture date.
What testing is performed on each batch?
Every batch undergoes: sterility testing (USP <71> for aerobic, anaerobic bacteria and fungi), mycoplasma detection (PCR and culture), hemoglobin quantification (≤60 mg/dL), total protein measurement (4.0-7.0 g/dL), endotoxin testing (≤50 EU/mL), pH verification (7.0-8.5), and osmolality measurement (260-340 mOsm/kg). Source animals undergo veterinary inspection and disease screening.
Can I request a Certificate of Analysis (COA)?
Absolutely! Every batch comes with a COA showing test results for all QC parameters. You can request the COA for your specific lot number before ordering by contacting us at info@seamlessbio.de or through our shop. We also provide Certificate of Origin (COO) and MSDS documentation.
Why Choose SeamlessBio?
🏆 Quality Assurance
ISO 9001 certified production with rigorous testing protocols and complete batch documentation for every shipment.
💰 Competitive Pricing
Direct sourcing and efficient operations allow us to offer excellent value without compromising on quality.
🚚 Reliable Delivery
Fast shipping across EU and internationally on dry ice with temperature monitoring. Most orders ship within 1-2 business days.
🔬 Expert Support
Technical team available to help with application optimization, protocol troubleshooting, and product selection.
📦 Flexible Options
Multiple sizes, treatment options (heat-inactivated, gamma-irradiated), and custom packaging available.
🌍 Dual Sourcing
Both USA and EU origin available to meet your regulatory requirements and regional preferences.
Ready to Order Species-Optimized Chicken Serum?
Get USA/EU-origin quality for your avian cell culture with fast delivery and expert support.
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Questions about which serum is right for you?
Phone: +49 851 37932226 | Email: info@seamlessbio.de