BSA Fraction V pH 5.2 — Acidic Buffer Applications & pH-Dependent Assays
Standard BSA Fraction V is supplied at pH 6.8–7.2. For applications that require albumin at acidic pH — or where the BSA must be dissolved and used directly without pH adjustment — BSA pre-adjusted to pH 5.2 eliminates the need for time-consuming buffer titration and the risk of protein aggregation during pH adjustment.
BSA pH 5.2 corresponds to approximately the isoelectric point (pI) region of albumin (~4.7–5.2), where electrostatic interactions with charged surfaces differ from neutral pH conditions. This property is exploited in specific immunoassay and protein chemistry applications.
Applications of BSA pH 5.2
| Application | Why pH 5.2 specifically? |
|---|---|
| Acidic blocking buffers for IHC/ELISA | Some assay systems use acidic wash or incubation buffers where standard pH 7 BSA would alter the buffer pH — pre-adjusted BSA maintains buffer composition |
| Protein A/G purification workflows | IgG elution from Protein A/G typically occurs at pH 3–4; re-equilibration buffers near pH 5 use pH 5.2 BSA as carrier |
| Lysosomal enzyme assays | Lysosomal assays run at pH 4.5–5.5; pH 5.2 BSA avoids precipitation and pH shift when added as substrate carrier |
| Acidic nanoparticle coating | Nanoparticle surface functionalisation at acidic pH uses pH 5.2 BSA to avoid aggregation during BSA corona formation |
| pH-dependent binding studies | FcRn receptor binds IgG at pH 6.0 and releases at pH 7.4 — comparative studies at acidic pH use pH 5.2 BSA as protein carrier |
Specifications
| Parameter | Specification |
|---|---|
| Purity | ≥96% (electrophoresis) |
| pH (10 mg/mL solution) | 5.0–5.4 |
| Form | Lyophilised powder |
| Origin | EU or US |
| Pack sizes | 10 g, 50 g, 100 g, 500 g | Bulk on request |
| Storage | +4 °C | −20 °C for long-term |
| Documentation | CoA (purity, pH), CoO, MSDS |
Complete BSA Portfolio at SeamlessBio
| BSA Grade | Key Properties | Primary Applications | Order |
|---|---|---|---|
| Standard Grade | ≥96% purity, Fraction V | General cell culture, protein assays, general blocking | Shop → |
| Low Endotoxin | ≤5 EU/mg, ≥98% | Primary cell culture, stem cells, sensitive assays | Shop → |
| Fatty Acid-Free | FA-free, ≥98% | Lipid/fatty acid research, membrane assays, drug binding | Shop → |
| pH 5.2 | ≥96%, adjusted to pH 5.2 | Acidic assay systems, pH-dependent binding studies | Shop → |
Contact: info@seamlessbio.de | +49 851 37932226