BSA Low Endotoxin — ≤5 EU/mg | Fraction V ≥98% | Cell Culture Grade

BSA Low Endotoxin is the correct grade when endotoxin contamination would impair cell viability, activation state, or assay readout. Endotoxins (lipopolysaccharides, LPS) from gram-negative bacterial contamination are potent activators of the innate immune response — at concentrations as low as 0.1 EU/mL they trigger NFκB signalling, cytokine release, and cell death in sensitive systems. Standard BSA grades contain endotoxin levels that make them unsuitable for primary cells, macrophages, dendritic cells, and immune assays.

Why Endotoxin Matters in Cell Culture

Endotoxins bind to TLR4/MD-2 complexes on immune and many non-immune cells. In cell culture, even sub-nanogram quantities trigger:

Effect	Consequence for experiments
NFκB activation	Non-specific cytokine secretion — confounds immunology assays
Cell stress response	Altered gene expression, differentiation artefacts in stem cells
Macrophage activation	False-positive inflammatory response in co-culture models
Reduced viability in sensitive lines	Unexplained primary cell death with standard BSA lots
Inhibited virus production	LPS suppresses viral replication in some production systems

Applications of BSA Low Endotoxin

Application	Protocol	Why low endotoxin?
Primary cell culture (hepatocytes, neurons, cardiomyocytes)	0.1–4 mg/mL in serum-free or reduced-serum medium	Primary cells are endotoxin-sensitive — standard BSA causes non-specific activation
Macrophage and dendritic cell culture	0.1–2 mg/mL as serum supplement	Macrophages respond to femtomolar LPS — low endotoxin BSA essential for baseline assays
PBMC stimulation assays	0.5–2 mg/mL as carrier in cytokine stimulation medium	Prevents LPS-driven background cytokine release in ELISpot and cytokine bead arrays
Serum-free medium formulation	Replacement for serum albumin in defined media	Low endotoxin BSA used in xeno-free and defined media for cell therapy applications
ELISA and immunoassay development	1–5% blocking buffer	Endotoxin in blocking buffer can activate NFκB reporter cell lines used in bioassays

Specifications

Parameter	Specification
Purity	≥98% (electrophoresis)
Endotoxin	≤5 EU/mg (LAL method)
Form	Lyophilised powder
pH (10 mg/mL solution)	6.8–7.2
Origin	EU, US or Australia/NZ
Pack sizes	5 g, 10 g, 50 g, 100 g, 500 g, 1 kg | Bulk on request
Storage	+4 °C (short-term) | −20 °C (long-term)
Documentation	CoA (endotoxin, purity, pH), CoO, MSDS

Complete BSA Portfolio at SeamlessBio

BSA Grade	Key Properties	Primary Applications	Order
Standard Grade	≥96% purity, Fraction V	General cell culture, protein assays, general blocking	Shop →
Low Endotoxin	≤5 EU/mg, ≥98%	Primary cell culture, stem cells, sensitive assays	Shop →
Fatty Acid-Free	FA-free, ≥98%	Lipid/fatty acid research, membrane assays, drug binding	Shop →
pH 5.2	≥96%, adjusted to pH 5.2	Acidic assay systems, pH-dependent binding studies	Shop →

Order BSA Low Endotoxin

Contact: info@seamlessbio.de | +49 851 37932226