Rat Serum (Normal Rat Serum) — IHC Blocking & Cell Culture | EU Origin | SeamlessBio
EU Stock0. 2 µm sterile filteredNo MOQCoA included
Normal Rat Serum is used as a species-matched blocking agent when anti-rat secondary antibodies are used in IHC, IF, and flow cytometry protocols. It is also the appropriate serum supplement for rat primary cell culture — particularly for rat hepatocytes, neurons, and cardiac myocytes — where species-specific proteins support more physiologically relevant culture conditions.
SeamlessBio supplies Rat Serum from EU stock — no MOQ, CoA, batch reservation available.
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Applications
| Application | Protocol | Why? |
|---|---|---|
| IHC / IF Blocking | 5% rat serum in PBS-T, 30–60 min RT | Prevents detection of endogenous rat IgG as non-specific signal |
| Flow Cytometry Blocking | 2–5% rat serum for Fc receptor blocking | Critical for CD16/32 blocking in murine/rat flow cytometry panels |
| Rat Primary Cell Culture | Hepatocytes, neurons, cardiac myocytes | Species-specific growth factors improve viability vs FBS |
| Rat ELISA Controls | Negative control matrix in anti-rat antibody ELISA | Validates assay specificity in rat model studies |
| In Vivo Model Validation | Serum matrix for ex vivo assay calibration | Pharmacology and toxicology studies using rat models |
Technical Specifications
| Parameter | Specification |
|---|---|
| Species | Rat (Rattus norvegicus) |
| Collection | Pooled healthy donors |
| Filtration | 0.2 µm sterile filtered |
| Origin | EU |
| Storage | −20 °C |
| Volumes | 100 mL, 500 mL | Bulk on request |
| Documentation | CoA, CoO on request |
| Options | Heat inactivated on request |
Frequently Asked Questions
What concentration of rat serum for Fc receptor blocking in flow cytometry?
2–5% rat serum for 10–15 minutes on ice before antibody staining. For CD16/32 blocking specifically, commercial anti-CD16/32 antibody can be used in addition.
Is heat inactivated rat serum available?
Yes — heat inactivated (56°C, 30 min) rat serum is available on request from SeamlessBio.
Complete Animal Serum Portfolio at SeamlessBio
All 9 animal sera from one EU source — no minimum order.
| Species | Abbr. | Primary Application |
|---|---|---|
| Rabbit | NRS | IHC/WB/ELISA blocking — anti-rabbit secondary antibodies |
| Goat | NGS | IHC/IF blocking — most common secondary antibody host |
| Sheep | NSS | Blocking, complement CFT, vaccine & vet diagnostics |
| Mouse | NMS | ELISA controls, anti-mouse IHC, primary cell culture |
| Rat | — | Anti-rat IHC blocking, rat primary cell culture |
| Horse | DHS | PC12 differentiation, hybridoma, muscle differentiation |
| Chicken | NCS | CEF cell culture, poultry ELISA controls |
| Pig | NPS | Swine vaccine development, porcine vet diagnostics |
| Guinea Pig | NGPS | CFT complement source — unique diagnostic niche |
Order Information
| Parameter | Details |
|---|---|
| Shop URL | shop.seamlessbio.de/en/products/rat-serum |
| Volumes | 100 mL, 500 mL | Bulk on request |
| Documentation | CoA, CoO on request — free with every order |
| Free test volume | Available before purchase — contact us |
| Batch reservation | Yes — reserve one lot for your entire project |
| info@seamlessbio.de | |
| Phone | +49 851 37932226 |