Antibody Production — Custom Sourcing & Partner Network
SeamlessBio acts as your single point of contact for custom antibody production projects. Rather than maintaining in-house production capacity, we work with a vetted network of specialist manufacturers across Europe — matching your project requirements to the most suitable production partner based on antibody type, format, scale, regulatory requirements, and timeline. You deal with SeamlessBio; we manage the supplier relationship.
How it works: You describe your project — target antigen, intended application, format, quantity, documentation requirements. We identify the most suitable production partner from our network, obtain quotes, manage the project coordination, and deliver the finished antibody with full documentation. No need to identify and qualify multiple CROs yourself.
What We Source for You
| Antibody Type | Production Method | Typical Applications |
|---|---|---|
| Monoclonal Antibodies (mAb) Mouse, rat, rabbit |
Hybridoma technology — immunisation, fusion, screening, clonal expansion, ascites or bioreactor production | ELISA, Western blot, IHC, flow cytometry, immunoprecipitation, diagnostic kit development |
| Recombinant Monoclonal Antibodies Human, humanised, chimeric |
Variable region sequencing from hybridoma or B cells → recombinant expression in CHO or HEK293 — defined sequence, reproducible manufacturing | Therapeutic development, clinical research, long-term reproducibility requirements, regulatory submissions |
| Polyclonal Antibodies Rabbit, goat, sheep, donkey |
Host immunisation with antigen → bleed schedule → affinity purification. Various adjuvant protocols available. | Research-grade detection, secondary antibody production, antigen characterisation, rapid feasibility studies |
| Single-Domain Antibodies (Nanobodies / VHH) | Camelid immunisation (llama, alpaca) → VHH library → phage display selection → E. coli or yeast expression | Structural biology (cryo-EM, X-ray), intrabodies, proximity labelling, diagnostic applications requiring small format |
| Bispecific Antibodies | Various formats: IgG-scFv, tandem scFv (BiTE), DART — recombinant production in CHO/HEK293 | Immuno-oncology research, T cell redirection, co-engagement assays |
| Antibody Fragments Fab, F(ab')₂, scFv |
Enzymatic digestion (papain/pepsin) or recombinant expression in E. coli or yeast | Reduced Fc-mediated background in assays, tissue penetration studies, conjugation applications |
| Antibody-Drug Conjugates (ADC) Research Reagents | Conjugation of purified mAb to payload (toxin, fluorophore, biotin) — site-specific or random conjugation | ADC research, drug delivery, flow cytometry panels, ELISA detection reagents |
Production Scales Available
| Scale | Typical Yield | Use Case |
|---|---|---|
| Pilot / Feasibility | 1–5 mg purified antibody | Initial validation, epitope confirmation, assay development before commitment to full production |
| Research Scale | 5–50 mg purified antibody | Standard research projects, assay panels, internal tool antibody generation |
| Mid-Scale | 50–500 mg purified antibody | Diagnostic kit manufacturing, larger research programmes, preclinical studies |
| GMP-Adjacent / GMP | Gram scale and above | IND-enabling studies, Phase I clinical material, regulated in vitro diagnostic manufacturing |
Services Along the Full Antibody Development Chain
| Stage | What We Can Source |
|---|---|
| Antigen production | Recombinant protein or peptide antigen synthesis for immunisation — if you do not have antigen available, we source this first |
| Immunisation & screening | Host animal immunisation, bleed schedules, ELISA screening of immune response, hybridoma fusion and single-cell cloning |
| Sequencing & recombinant conversion | Variable region sequencing from hybridoma → vector cloning → transient or stable expression in CHO/HEK293 for defined, reproducible manufacturing |
| Purification | Protein A/G affinity chromatography, size exclusion, ion exchange — specified purity grade, aggregation testing, endotoxin removal |
| Characterisation | SDS-PAGE, SEC-HPLC, ELISA, SPR (Biacore) kinetics, epitope mapping, species cross-reactivity panel, functional assay validation |
| Formulation & fill | Buffer exchange to project-specific formulation, sterile filtration, aliquoting, lyophilisation — QC release with CoA |
| GMP documentation | Batch records, in-process testing, release testing, ICH Q6B compliance, regulatory support for IND submissions |
How the Process Works
1. Project briefing — You send us your project requirements: target, application, format, quantity, timeline, documentation needs. A brief email or call is sufficient.
2. Partner matching — We identify the most suitable production partner from our network based on your specific requirements. We obtain quotes and technical proposals on your behalf.
3. Proposal to you — You receive a single consolidated proposal from SeamlessBio covering timeline, scope, price, and documentation deliverables. No need to coordinate multiple suppliers.
4. Project execution — SeamlessBio manages the production partner relationship. You receive regular project updates. We escalate any technical issues directly.
5. Delivery — Finished antibody delivered with full documentation package: CoA, purity data, characterisation data, and any project-specific regulatory documents.
What We Need From You to Provide a Quote
| Information | Details |
|---|---|
| Target antigen | Protein name, species, accession number or sequence. If antigen production is needed, specify domain/epitope of interest. |
| Antibody type | Monoclonal / polyclonal / recombinant / fragment. Host species preference if applicable. |
| Intended application | ELISA, WB, IHC, flow cytometry, functional blocking, therapeutic research — determines screening strategy |
| Quantity required | Milligrams of purified antibody needed — pilot (1–5 mg), research (5–50 mg), larger scale |
| Timeline | Desired delivery date or project deadline |
| Documentation requirements | Research use only (CoA) / diagnostic kit (extended characterisation) / GMP (batch records, release testing) |
| Budget indication | Optional — helps us identify the most cost-effective partner for your requirements |
Why Use SeamlessBio for Antibody Sourcing?
| Advantage | Details |
|---|---|
| Single point of contact | One relationship, one contract, one invoice — regardless of which production partner executes the project |
| Pre-qualified partner network | We work with established European manufacturers — you benefit from our supplier qualification without doing it yourself |
| Project management included | We manage timelines, technical communication, and issue resolution — reducing your project management burden |
| Flexibility | Access to multiple production platforms (hybridoma, recombinant, phage display) through one contact — matched to your project |
| Reagent supply integration | We also supply FBS, human serum, BSA, and cell culture media needed for production — consolidating your supplier base |
Related Products for Antibody Production
| Product | Relevance | Link |
|---|---|---|
| FBS Ultra Low IgG | Hybridoma culture and mAb production — eliminates bovine IgG contamination in supernatant | View → |
| FBS Low Endotoxin | Sensitive hybridoma lines, primary B cell culture | View → |
| Human Serum Type AB Male | Human B cell culture, EBV immortalisation, human hybridoma protocols | View → |
| BSA Low Endotoxin | Blocking agent in ELISA, conjugation buffer, antibody storage buffer | View → |
Send your project requirements to info@seamlessbio.de or call us at +49 851 37932226. We respond within 1 business day with an initial assessment and timeline for a full proposal.