ciPTEC – The Gold Standard Human Renal Cell Line for Nephrotoxicity & DDI Research
Conditionally immortalized human proximal tubule epithelial cells with stable expression of all FDA/EMA-relevant drug transporters for 20+ passages. Used in 30+ laboratories. 90+ peer-reviewed publications.
ciPTEC Cell Line Portfolio
Three validated human renal proximal tubule cell line variants for every stage of preclinical drug development – from nephrotoxicity screening to full FDA/EMA transporter DDI characterization.
The foundational human proximal tubule cell line. Endogenous expression of key renal transporters. Stable for 20+ passages. Base model for general nephrotoxicity, cell viability, and transporter activity assays.
Request & Order →ciPTEC Parental stably transfected with functional OAT1 (SLC22A6). The most comprehensive renal in vitro model available. Validated in collaboration with AstraZeneca: 75% sensitivity, 100% specificity across 62 compounds.
Request & Order →ciPTEC Parental stably transfected with functional OAT3 (SLC22A8). Ideal for basolateral organic anion uptake studies and complete renal transport characterization alongside ciPTEC-OAT1.
Request & Order →ciPTEC vs. Alternatives
Based on published data, conference presentations, and Cell4Pharma internal validation. The key differentiator: stable, functional OAT1/OAT3/OCT2 expression – a combination no competitor reliably provides.
| Feature | ciPTEC ✓ Best | HK-2 (ATCC) | Primary PTECs | SA7K (Merck) | RPTEC/TERT (Evercyte) |
|---|---|---|---|---|---|
| Human proximal tubule origin | ✔ | ~ HPV-transformed | ✔ | ✔ | ✔ |
| Stable functional OAT1 | ✔ Functional | ✗ Lost | ~ Hours only | ✗ Absent | ~ Inconsistent |
| Stable functional OAT3 | ✔ Functional | ✗ | ~ Hours only | ✗ | ✗ Low |
| Endogenous OCT2 | ✔ | ✗ | ~ | ✗ | ✗ Low |
| P-gp / MRP2 / MRP4 / BCRP | ✔ All functional | ~ Reduced | ~ | ~ | ~ |
| Stable for 20+ passages | ✔ | ✔ | ✗ Days only | ✔ | ✔ |
| FDA/EMA DDI full transporter panel | ✔ Complete | ✗ | ✗ | ✗ No OATs | ~ Partial |
| 96-well HTS compatible | ✔ | ✔ | ✗ | ✔ | ✔ |
| No donor-to-donor variation | ✔ | ✔ | ✗ High | ✔ | ✔ |
| No proprietary medium required | ✔ | ✔ | ✔ | ✔ | ✗ Evercyte only |
| Peer-reviewed publications | 90+ | Many (limited tox use) | Many | <10 | Limited |
| Transfer to customer labs | ✔ Full | ✔ | ✗ | ✔ | ✔ |
Sources: Jenkinson et al. (2012) – HK-2 limitations | Vormann et al. AAPSJ (2018) – SA7K | Cell4Pharma internal validation | Published conference presentations
Why ciPTEC is the Right Choice for Your Lab
The only human renal cell line with stable, validated expression of the complete FDA/EMA renal transporter panel – developed over a decade of academic-industry collaboration.
ciPTEC-OAT1 expresses OAT1, OCT2, P-gp, MRP2, MRP4, and BCRP. No competitor provides this full panel in a single transferable cell line without proprietary medium requirements.
All transporters listed in FDA (2017) and EMA (2013) DDI guidelines for renal studies are covered. Run your complete preclinical transporter package in one validated model.
Stable for 20+ passages means your entire organization runs identical assays from the same cell stock. No donor variation. No batch inconsistency. No fee-for-service bottleneck.
No other transferable human renal cell line for nephrotoxicity screening has this publication depth. Used in 30+ laboratories across pharma, biotech, CRO, and academia worldwide.
96-well format compatible. Multi-parametric readouts validated: cytoskeleton (Phalloidin), mitochondria (MitoTracker), nuclear (DAPI). 75% sensitivity, 100% specificity.
SeamlessBio ships from European stock with EUR invoicing, full CoA documentation, and technical support in German and English. No customs delays, no hidden costs.
Validated Assays in ciPTEC Models
Over a decade of validation with pharmaceutical partners. Limited inter-laboratory variation across all assay formats.
ciPTEC – Technical & Ordering FAQ
Common questions from ADME-tox researchers, process development scientists, and procurement.
Ready to upgrade your renal toxicity model?
Request ciPTEC cells, technical documentation, or a free consultation. SeamlessBio ships to all DACH countries – EUR invoicing, full GMP documentation, direct support.