Mouse Serum (Normal Mouse Serum, NMS) — ELISA Control & IHC Blocking | EU Origin | SeamlessBio
EU Stock0.2 µm sterile filteredNo MOQCoA included
Normal Mouse Serum (NMS) is essential in two major workflows: as a species-matched blocking agent when anti-mouse secondary antibodies are used in IHC and IF, and as a negative control serum in mouse antibody ELISA panels. It is also the preferred serum supplement for mouse primary cell culture.
SeamlessBio supplies Mouse Serum from EU stock — no MOQ, CoA, batch reservation available.
🛒 Order Mouse Serum now
Applications of Normal Mouse Serum (NMS)
| Application | Standard Protocol | Why NMS? |
|---|---|---|
| IHC / IF Blocking | 5% NMS in PBS-T, 30–60 min RT before anti-mouse secondary | Prevents detection of endogenous mouse IgG in tissue as non-specific signal |
| ELISA Negative Control | NMS as negative matrix control in sandwich ELISA | Validates specificity of anti-mouse capture/detection antibody pairs |
| Mouse Primary Cell Culture | 5–10% NMS in DMEM for MEF, neurons, hepatocytes | Species-specific growth factors improve viability vs FBS |
| Hybridoma Culture | NMS in HAT selection medium formulation | Supports murine hybridoma expansion post-fusion |
| Isotype Control Diluent | NMS as isotype control background | Matches immunoglobulin background for control antibody experiments |
Technical Specifications
| Parameter | Specification |
|---|---|
| Species | Mouse (Mus musculus) |
| Abbreviation | NMS — Normal Mouse Serum |
| Collection | Pooled healthy donors |
| Filtration | 0.2 µm sterile filtered |
| Origin | EU |
| Storage | −20 °C |
| Volumes | 100 mL, 500 mL | Bulk on request |
| Documentation | CoA, CoO on request |
Frequently Asked Questions
Why is mouse serum used as blocking agent in IHC?
Tissues contain endogenous mouse IgG. Anti-mouse secondary antibodies detect this endogenous IgG as non-specific signal. Blocking with NMS saturates these sites before adding the secondary antibody.
Can NMS replace FBS in mouse primary cell culture?
For species-specific applications like MEF and mouse neuron culture, NMS provides more physiologically relevant growth signals than FBS. Commonly used at 5–10% in DMEM.
Complete Animal Serum Portfolio at SeamlessBio
| Species | Abbr. | Primary Application |
|---|---|---|
| Rabbit | NRS | IHC/WB/ELISA blocking — anti-rabbit secondary antibodies |
| Goat | NGS | IHC/IF blocking — most common secondary antibody host |
| Sheep | NSS | Blocking, complement CFT, vaccine & vet diagnostics |
| Mouse | NMS | ELISA controls, anti-mouse IHC, primary cell culture |
| Rat | — | Anti-rat IHC blocking, rat primary cell culture |
| Horse | DHS | PC12 differentiation, hybridoma, muscle differentiation |
| Chicken | NCS | CEF cell culture, poultry ELISA controls |
| Pig | NPS | Swine vaccine development, porcine vet diagnostics |
| Guinea Pig | NGPS | CFT complement source — unique diagnostic niche |
Order Information
| Parameter | Details |
|---|---|
| Shop URL | shop.seamlessbio.de/en/products/mouse-serum |
| Volumes | 100 mL, 500 mL | Bulk on request |
| Documentation | CoA, CoO on request — free with every order |
| Free test volume | Available before purchase — contact us |
| Batch reservation | Yes — reserve one lot for your entire project |
| info@seamlessbio.de | |
| Phone | +49 851 37932226 |