Chicken Serum (Normal Chicken Serum, NCS) — CEF Cell Culture & Poultry Diagnostics | EU Origin | SeamlessBio
EU Stock0. 2 µm sterile filteredNo MOQCoA included
Normal Chicken Serum (NCS) serves three distinct roles: as a blocking agent when anti-chicken secondary antibodies are used in IHC and ELISA, as a supplement for chicken embryo fibroblast (CEF) cell culture in avian virology and vaccine production, and as a negative control matrix in poultry diagnostic ELISA kits (NDV, Marek's, IBD).
Applications
| Application | Protocol | Why? |
|---|---|---|
| IHC / ELISA Blocking | 5% NCS before anti-chicken secondary antibodies | Essential for IHC panels using chicken primary antibodies |
| CEF Cell Culture | 10% NCS in MEM for chicken embryo fibroblast culture | NDV, MDV, IBV propagation — species-specific serum superior to FBS |
| Poultry Diagnostic ELISA | NCS as negative control matrix in NDV, IBD, Marek's ELISA kits | Standard negative control for poultry serology |
| Avian Virology | NCS supplement for avian cell lines (MDCK, DF-1, CEC-32) | Species-specific growth factors, hormones, and lipoproteins |
| HI Assay Background Control | Non-immune NCS as seronegative control in HI titration | Hemagglutination inhibition assays for avian paramyxoviruses |
Technical Specifications
| Parameter | Specification |
|---|---|
| Species | Chicken (Gallus gallus domesticus) |
| Abbreviation | NCS — Normal Chicken Serum |
| Collection | Pooled SPF or monitored flocks |
| Filtration | 0.2 µm sterile filtered |
| Origin | EU |
| Storage | −20 °C |
| Volumes | 100 mL, 500 mL | Bulk on request |
| Documentation | CoA, CoO on request |
Frequently Asked Questions
Why is chicken serum preferred over goat serum for CEF cell culture?
Chicken embryo fibroblasts are avian cells — species-specific serum provides avian growth factors, hormones, and lipoproteins that better support CEF attachment, proliferation, and virus susceptibility.
Can chicken serum be used for Newcastle Disease ELISA controls?
Yes — non-immune NCS from unvaccinated NDV-seronegative flocks is the standard negative control matrix in NDV ELISA kits. Lot documentation available on request.
Complete Animal Serum Portfolio at SeamlessBio
All 9 animal sera from one EU source — no minimum order.
| Species | Abbr. | Primary Application |
|---|---|---|
| Rabbit | NRS | IHC/WB/ELISA blocking — anti-rabbit secondary antibodies |
| Goat | NGS | IHC/IF blocking — most common secondary antibody host |
| Sheep | NSS | Blocking, complement CFT, vaccine & vet diagnostics |
| Mouse | NMS | ELISA controls, anti-mouse IHC, primary cell culture |
| Rat | — | Anti-rat IHC blocking, rat primary cell culture |
| Horse | DHS | PC12 differentiation, hybridoma, muscle differentiation |
| Chicken | NCS | CEF cell culture, poultry ELISA controls |
| Pig | NPS | Swine vaccine development, porcine vet diagnostics |
| Guinea Pig | NGPS | CFT complement source — unique diagnostic niche |
Order Information
| Parameter | Details |
|---|---|
| Shop URL | shop.seamlessbio.de/en/products/chicken-serum |
| Volumes | 100 mL, 500 mL | Bulk on request |
| Documentation | CoA, CoO on request — free with every order |
| Free test volume | Available before purchase — contact us |
| Batch reservation | Yes — reserve one lot for your entire project |
| info@seamlessbio.de | |
| Phone | +49 851 37932226 |