Custom Cell Culture Media Production Tailored to Your Specifications
Custom Cell Culture Media Solutions for Research, Development and Industrial Production
ISO-certified manufacturing | Flexible formulations | GMP support | Bulk production
Challenges with Standard Cell Culture Media?
Standard media don't fit your specific cell type
Off-the-shelf media deliver suboptimal results for your specialized cell lines or primary cells
Insufficient batch sizes for scale-up
Commercial standard sizes are inadequate for your production processes or clinical trials
Missing GMP documentation
Regulatory requirements for CGT and pharma production cannot be met
No control over media components
Special requirements like serum-free formulations, SILAC, or component exclusions impossible
Inconsistent batch quality
Variability between batches affects reproducibility of your experiments
Long delivery times for specialty products
Delays in critical project phases due to inflexible supply chains
Your Solution: Custom Cell Culture Media from SeamlessBio
As a specialized provider of biological reagents, we offer completely customized cell culture media solutions with the highest quality standards. Our ISO 9001:2015-certified partners enable flexible adaptations for any application.
100% Customizable Formulations
Every medium according to your exact specifications: component adjustment, serum-free, chemically defined, or SILAC media
Flexible Batch Sizes
From research quantities (starting at 1L) to industrial large batches (up to 1000L+) – scalable as needed
GMP Compliance & Complete Documentation
cGMP-compliant production with complete regulatory documentation for clinical applications
Consistent Batch Quality
Strict QC procedures guarantee batch-to-batch reproducibility
Fast Development & Delivery
Agile media development with typical turnaround times of 4-8 weeks for first batches
Technical Support & Optimization
Scientific consulting for media optimization and process development by experienced cell culture experts
Our Custom Media Services in Detail
Our Process: From Request to Delivery
Step 1: Request & Needs Analysis
Duration: 1-2 business days
- Capture your specific requirements
- Analysis of cell type, application and volume
- Discussion of regulatory requirements
- Technical feasibility assessment
Step 2: Quote & Formulation Design
Duration: 3-5 business days
- Detailed quote with cost breakdown
- Proposed media formulation
- Definition of QC tests and specifications
- Timeline for development and production
Step 3: Media Development & Prototyping
Duration: 2-4 weeks
- Manufacturing of test batches (optional)
- Functional validation with your cells
- Iterative optimization based on feedback
- Finalization of formulation
Step 4: Qualification & Documentation
Duration: 1-2 weeks
- Creation of master batch documentation
- Development of standard operating procedures
- Execution of qualification runs
- Generation of QC documentation
Step 5: Production & QC Release
Duration: 2-3 weeks
- Production of ordered batch size
- Execution of all agreed QC tests
- Review and release by quality assurance
- Creation of CoA and shipping documentation
Step 6: Delivery & Support
Duration: 2-5 business days
- Temperature-controlled shipping
- Tracking and delivery confirmation
- Technical support for application questions
- Long-term availability for re-orders
Typical total duration: 6-12 weeks from request to first delivery
Re-order lead time: 2-4 weeks for established formulations
Application Areas for Custom Cell Culture Media
Pharmaceutical & Biopharmaceutical Production
Cell & Gene Therapy (CGT)
Academic Research & Drug Discovery
Diagnostics & In Vitro Testing
- Recombinant protein production: CHO, HEK293 production media for monoclonal antibodies
- Viral vector production: Media for AAV, lentivirus, adenovirus manufacturing
- Vaccine production: Cell-based vaccine manufacturing media
- Biosimilar development: Comparability-compliant media formulations
- CAR-T cell expansion: GMP-grade media for T-cell expansion and transduction
- MSC therapy: Mesenchymal stem cell media for regenerative medicine
- iPSC-based therapies: Pluripotent stem cell media with defined composition
- Tissue engineering: Specialized media for tissue cultivation and 3D constructs
- Primary cell studies: Optimized media for human primary cells from diverse tissues
- Disease modeling: Disease-specific media for pathological studies
- High-throughput screening: Consistent media for compound screening campaigns
- Organoid research: Complex media for 3D organoids from various tissues
- Companion diagnostics: Standardized media for diagnostic assays
- Toxicity testing: Defined media for hepatotoxicity, cardiotoxicity, etc.
- Efficacy testing: Consistent media for drug efficacy testing
- Quality control: Cell-based assay media for biopharma QC
Success Stories: Custom Media in Practice
Case 1: CAR-T Cell Therapy Scale-up
Case 2: iPSC Organoid Platform
Case 3: mAb Production Optimization
Challenge: A biotech company needed a serum-free, GMP-grade medium for commercial CAR-T cell production with consistent batch-to-batch performance.
Solution: Development of a chemically defined T-cell expansion medium with optimized cytokine concentrations, produced in 100L batches with complete cGMP documentation.
Result: 3-fold higher T-cell expansion compared to starting medium, <0.5% batch-to-batch variability, successful IND submission.
Challenge: Research institute sought custom medium for liver organoids from iPSCs with reduction of serum content for better differentiation.
Solution: Custom formulation with reduced FBS (2% instead of 10%), supplemented with specific growth factors (HGF, OSM, dexamethasone) and matrix components.
Result: Improved hepatocyte marker expression (+40%), enhanced functional maturity (albumin secretion +2.5x), reduced media costs by 30%.
Challenge: Pharma company wanted CHO production medium with higher titer and extended production phase for monoclonal antibodies.
Solution: DoE-based media optimization with increased amino acid concentration, anti-apoptotic factors and fed-batch-compatible supplements, scale-up to 500L.
Result: Titer increase of 60% (from 2.5 to 4.0 g/L), extension of production phase by 3 days, improvement of product quality (reduced aggregates).
Our Quality Assurance Measures
- Validated methods: All test methods validated according to ICH guidelines
- Raw material testing: Incoming QC for all raw materials with CoA review
- Environmental monitoring: Continuous monitoring of cleanroom environments
- Equipment qualification: IQ/OQ/PQ for all critical production equipment
- Change control: Documented management of all process changes
- Deviation management: CAPA system for systematic deviation handling
- Annual product review: Yearly review of all products for trend analysis
- Supplier qualification: Audit and qualification of all critical suppliers
Quality & Certifications
ISO 9001:2015
Certified quality management system for consistent product quality and continuous improvement
ISO 13485
Medical device QM system for regulated production of media for clinical applications
cGMP Compliance
Current Good Manufacturing Practice according to FDA 21 CFR Part 211 and EU GMP Annex 2
GLP Compatible
Good Laboratory Practice-compatible documentation for preclinical studies
Pricing & Minimum Order Quantities
The costs for custom cell culture media depend on several factors:
- Formulation complexity: Number and cost of components, availability of GMP-grade materials
- Production volume: Larger batches have lower costs per liter through economies of scale
- QC requirements: Standard tests vs. extended analytics and functional validation
- Documentation level: Research-grade vs. GMP documentation package
- Development effort: Existing formulation vs. de-novo media development
- Delivery frequency: One-time order vs. regular supply contracts
Indicative Pricing for Typical Scenarios
Research-Grade Custom Media (Standard Formulation)
Batch size: 10-50 liters | Price per liter: €150-300
Includes: Standard formulation based on established basal medium, standard QC tests, research-grade documentation
GMP-Grade Custom Media
Batch size: 50-200 liters | Price per liter: €400-800
Includes: cGMP production, extended QC, complete regulatory documentation (DMF support, CoA, CoO, etc.)
De-novo Media Development
Development fee: €5,000-25,000 (one-time)
Includes: Formulation design, prototyping (3-5 test batches), DoE optimization, functional validation, process development
Bulk Production (>500L)
Price per liter: On request (individual price negotiation)
Economies of scale for large-volume customers, long-term supply agreements possible
Minimum Order Quantities (MOQ)
- Research-grade: 5 liters minimum
- GMP-grade: 20 liters minimum (due to increased documentation effort)
- Development projects: Commitment to at least 50L production volume after development completion
Request an individual quote: Contact us with your specifications for a detailed price quote including timeline.
Frequently Asked Questions (FAQ)
What documentation do I receive with GMP-grade media?
Complete documentation package including: Certificate of Analysis (CoA), Certificate of Origin (CoO), BSE/TSE Statement, Viral Safety Statement, Statement on Microbial Agents, Statement of GMP Compliance, and optional Drug Master File (DMF) support.
Can you replicate existing media from other manufacturers?
Yes, we can perform reverse engineering or develop comparable media based on published formulations. Please note possible IP rights for proprietary media from other manufacturers.
Do you provide technical support after delivery?
Yes, we offer ongoing technical support for all custom media customers. Our scientists are available for performance issues or optimization questions.
How does re-ordering work if I want to order again?
Your formulation is saved in our database. Re-orders have shorter lead times (2-4 weeks) and you receive guaranteed identical composition. We proactively inform you of changes to raw materials.
Can you deliver internationally?
Yes, we deliver worldwide with temperature-controlled shipping. We handle all export formalities and provide necessary customs documents.
How long does it take to receive my first custom medium?
For modifications based on standard formulations: typically 4-6 weeks. For completely new media developments with optimization: 8-12 weeks. Express service for urgent projects available upon consultation.
Can I get small test quantities before ordering larger batches?
Yes, we even recommend pilot batches (typically 5-10L) for validation in your laboratory before committing to larger production runs. Costs for pilot batches are credited towards follow-up orders.
How is batch-to-batch consistency ensured?
Through strict QC specifications, qualified suppliers for raw materials, validated manufacturing processes, and functional tests with reference cell lines. Typical variability <3% for critical parameters.
Can you produce very small quantities (e.g., 100ml)?
Yes, for development projects or special applications we can also produce very small batches. Minimum quantity 100ml, however with higher relative costs per ml due to handling effort.
Is the formulation of my custom medium confidential?
Absolutely. We treat all customer formulations as highly confidential and are happy to sign NDAs before project start. Your formulation will never be shared with third parties.
Why SeamlessBio for Custom Cell Culture Media?
Scientific Expertise
Access to partner network with many years of experience in media development
Certified Quality
ISO 9001:2015 and ISO 13485 certified production partners with complete traceability
Fast EU Delivery
Own warehouse in Germany for fast availability and flexible logistics
Flexible Solutions
From small research quantities to industrial large batches – scalable as needed
Personal Consulting
Direct contact with experts for optimal solution finding
Complete Documentation
CoA, MSDS, BSE/TSE statements and GMP support for regulated applications
Cold-Chain Expertise
Temperature-controlled logistics for safe delivery of biological products
Global Network
Access to international manufacturers and quality standards
Ready for Your Custom Cell Culture Medium?
Let's develop the perfect media solution for your application together.