Fetal Bovine Serum – Low & Very Low Endotoxin
Reliable results in sensitive cell culture applications require serum with minimized endotoxin levels.
At SeamlessBio, we supply Fetal Bovine Serum (FBS) in Low Endotoxin and Ultra / Very Low Endotoxin quality – specially developed for demanding research and production processes.
Product Features
FBS Low Endotoxin
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Endotoxin level: < 5 EU/mL
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Ideal for standard cell culture and research applications
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Certified sterile-filtered, heat-inactivated or gamma-irradiated options
FBS Very Low Endotoxin
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Endotoxin level: < 1 EU/mL
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Recommended for sensitive cell lines, vaccine production, immunology & immunoglobulin research
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Ensures maximum reproducibility and reduced variability
Quality Standards
✅ Produced under cGMP & ISO 9001/13485 guidelines
✅ Batch-to-batch consistency and full traceability
✅ Documentation: Certificate of Analysis (COA), Certificate of Origin (COO), MSDS
✅ Long-term storage stability at –20 °C
Applications
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Vaccine and viral vector production (e.g., AAV, ADV)
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Immunoglobulin and antibody research
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Stem cell and regenerative medicine
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Diagnostics and clinical assay development
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Sensitive primary and hybridoma cell cultures
Packaging & Supply
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Available in 50 mL, 100 mL, 500 mL, and bulk volumes
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Custom packaging upon request
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Global delivery with cold chain logistics
Why SeamlessBio?
At SeamlessBio, we go beyond sourcing – we deliver quality, compliance, and reliability.
Our mission is to ensure that researchers and manufacturers receive materials that are not only high-performing, but also fully documented for regulatory and QA needs.
Request Your Quote Today
Looking for Fetal Bovine Serum with low or very low endotoxin levels?
Contact us for pricing, bulk orders, or to request a sample for evaluation.
Phone: +49 851 37932226
E-Mail to: info@seamlessbio.de
Frequently Asked Questions: FBS Low & Very Low Endotoxin
Everything you need to know about our low endotoxin fetal bovine serum
- Low Endotoxin FBS: Contains less than 5 EU/mL (Endotoxin Units per milliliter)
- Very Low Endotoxin FBS: Contains less than 1 EU/mL
- Hybridoma cells for antibody production
- Primary immune cells (lymphocytes, T cells, B cells)
- Dendritic cells and macrophages
- Stem cells (MSCs, iPSCs) in regenerative medicine
- CHO, HEK293, and Vero cells for protein production
- Cell lines for AAV and viral vector production
For all cell lines that are sensitive to bacterial contamination or immune stimulators, Low Endotoxin FBS is the preferred choice.
Find FBS for your cell line →- Store at -20°C for up to 10 years shelf life
- For maximum stability, store at -80°C (optional)
- Avoid direct sunlight and temperature fluctuations
- Use at 2-8°C in refrigerator for maximum 4 weeks
- Avoid repeated freezing and thawing (leads to protein denaturation)
- Thaw overnight at 2-8°C or in 37°C water bath (with constant swirling)
Documentation for GMP compliance:
- Certificate of Analysis (COA) – detailed quality testing of each lot
- Certificate of Origin (COO) – proof of origin
- MSDS (Material Safety Data Sheet)
- TSE/BSE compliance according to EU and FDA guidelines
- Viral safety according to 9 CFR (Code of Federal Regulations)
All lots are fully traceable and delivered with complete documentation.
Order GMP-compliant lots →| Test | Method | Specification |
|---|---|---|
| Endotoxin Test | LAL (Limulus Amebocyte Lysate) | <5 EU/mL (Low) / <1 EU/mL (Very Low) |
| Sterility | 14-day USP Sterility Test | Negative |
| Mycoplasma | PCR & Culture Test | Negative |
| Hemoglobin | Spectrophotometry | <25 mg/dL |
| Total Protein | Bradford Assay | 3.0-5.0 g/dL |
| pH Value | pH Meter | 6.8-8.0 |
| Osmolality | Freezing Point Depression | 260-320 mOsm/kg |
Additionally, each lot is tested for its growth promotion on relevant cell lines.
- All animals come from USDA-approved herds
- No feeding with animal proteins (prion risk excluded)
- Regular veterinary monitoring
- Complete traceability to the herd
All lots are tested for viral safety according to EU Regulation 999/2001 and FDA 9 CFR.
- Sterile Filtration (0.1 µm): Standard option, preserves all growth factors
- Heat-inactivated (56°C, 30 min): Inactivates complement factors, ideal for immunological assays
- Gamma-irradiated: Additional security for GMP production, reduces viral contamination
Recommendation: For most cell culture applications, sterile filtration is sufficient. We only recommend heat inactivation for specific requirements, as some growth factors may be lost in the process.
View all treatment options →- 50 mL – ideal for initial testing and small experiments
- 100 mL – standard size for routine laboratory applications
- 500 mL – optimal size for regular cell culture work
- Bulk volumes (5 L, 10 L, 20 L) – for industrial production and bioprocessing
- Endotoxin level (Low vs. Very Low)
- Treatment (sterile filtration, heat-inactivated, gamma-irradiated)
- Packaging size
- Order quantity
Volume discounts: Yes, we offer attractive tiered pricing starting from 5 liters. For long-term supply agreements and bulk purchasers, we create individual quotations.
- Germany: 1-2 business days (express option available)
- EU: 2-4 business days
- International: 5-7 business days
Cold-chain logistics: All FBS deliveries are made on dry ice with temperature monitoring. If needed, we also organize same-day delivery for urgent orders.
Our sample program:
- Free 50 mL test samples for qualified research institutions
- Complete COA documentation included
- Technical support during evaluation
- Possibility to test multiple lots in parallel
Request your sample directly through our shop or contact our sales team.
Request test sample →- Phone: +49 851 37932226 (Mon-Fri, 9 AM-5 PM CET)
- Email: info@seamlessbio.de
- Live Chat: Available in our online shop
- Optimizing FBS concentration for your cell line
- Troubleshooting unexpected cell culture problems
- Comparing different FBS qualities
- Adapting to new regulatory requirements
For legitimate quality complaints, we naturally offer replacement or full refund.
Ready to Order Your Low Endotoxin FBS?
Benefit from ISO 13485-certified quality, complete documentation, and fast delivery.
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Or contact us for an individual quotation:
Tel: +49 851 37932226 | Email: info@seamlessbio.de