Adeno-associated virus (AAV) vectors are critical to gene therapy. Their use in delivering genetic material into target cells has transformed modern medicine, from rare disease treatment to oncology and vaccine development. But producing AAVs isn't simple. It requires precise upstream and downstream processes — and most importantly, reliable biological raw materials.

Seamless Bio provides a portfolio of critical components used at every stage of AAV production. Whether you're working in early-stage R&D or GMP-grade manufacturing, our products help ensure consistency, scalability, and compliance.

1. Cell Expansion: High-Quality FBS for Consistent Growth

The AAV production process typically begins with the cultivation of HEK293 or similar cell lines. These cells require a dependable source of nutrients to proliferate and maintain viability prior to transfection.

Our solution:

• Premium Fetal Bovine Serum (FBS) from EU origin

• Available in heat-inactivated, gamma-irradiated, or low-endotoxin grades

• CoA and origin documentation included

🔗 Learn more: https://www.seamlessbio.de/products/human-and-animal-blood-products/fetal-bovine-serum-fbs

By offering lot reservation and free warehousing, we ensure your validated FBS supply remains consistent across batches — helping you avoid time-consuming requalification.

2. Transfection: Consistency That Translates Into Yield

Transient transfection is the heart of AAV production. Inconsistent serum or albumin quality at this stage can dramatically affect transfection efficiency and, in turn, viral yield.

Recommended products:

• Recombinant Human Serum Albumin (rHSA): animal-free, consistent, and GMP-ready

• Human Plasma / Human Serum: used in defined or customized transfection systems

rHSA is a preferred excipient and stabilizer for serum-free media. It ensures batch-to-batch consistency and is compliant with global regulatory standards.

🔗 rHSA details: https://www.seamlessbio.de/products/albumin/recombinant-human-serum-albumin

3. Harvest & Purification: Stabilizing Viral Particles

Once AAV particles are harvested, they're purified through filtration, chromatography, or tangential flow systems. Throughout these steps, biological additives play a key role in maintaining the integrity of the viral particles.

Seamless Bio provides:

• rHSA as a formulation stabilizer in purification buffers

• Human plasma proteins to simulate physiological conditions

These supplements help prevent AAV aggregation and degradation, especially during high-throughput or GMP-compliant processing.

4. Formulation & Final Fill: Ensuring Long-Term Stability

AAVs are sensitive to shear stress, temperature, and oxidation. Stabilizing agents in the formulation buffer are essential for product shelf life and safety.

Our recombinant HSA is ideal for this:

• Virus-free and animal-free

• Stable across pH and temperature fluctuations

• Supplied sterile, with full batch documentation

Available in volumes from 10 mL test vials to multi-liter bulk batches, our rHSA ensures performance and security at the most critical stage.

5. Single-Use Labware & Logistics: A Complete System

Beyond biologicals, we also support your cleanroom and lab operations with:

• Single-use bags for media prep and harvest

• Sterile bottles and filtration assemblies

• Custom labeling, packaging, and delivery planning

Every product comes with traceability documentation, CE certification (where applicable), and seamless integration into closed bioprocessing systems.

🔗 Explore labware: https://www.seamlessbio.de/products/lab-ware-and-bags

Why Seamless Bio?

We combine deep product knowledge with supply chain agility. Based in Germany, Seamless Bio offers:

• Fast EU delivery without customs delays

• Flexible quantities and packaging

• Dedicated account managers

• Technical support for qualification and validation

Whether you need a single test vial or long-term GMP supply contracts, we deliver the right material at the right time.

Ready to Optimize Your AAV Workflow?