Custom GMP-Ready Media for 293 Cell Expression—Quality Without Compromise

Recombinant protein production in HEK293 cells has become a cornerstone of modern biotechnology. Whether for vaccine development, antibody production, or gene therapy vectors such as AAVs, the scalability and consistency of 293-based expression systems make them indispensable.

But to ensure reproducibility and regulatory compliance, every component of the workflow must meet high quality standards – and that starts with the culture medium.

At Seamless Bio, we now offer custom GMP-ready media for 293 adherent and suspension cells. Produced in GMP-compliant facilities, our media deliver high performance, lot-to-lot consistency, and flexible configurations – at pricing that’s often significantly more competitive than existing market leaders.

✅ Why Custom GMP-Ready Media Matters

When working with 293 cells in a regulated environment or for therapeutic applications, using standard research-grade media can introduce unnecessary risk.

Key pain points include:

• Lot-to-lot variability

• Unknown animal-derived components

• Lack of traceability

• Import/export delays or customs barriers

• Overpriced catalog products without flexible packaging

Seamless Bio addresses these issues by offering:

• Chemically defined or protein-free formulations

• GMP-ready documentation (COA, BSE/TSE-free, ISO standards)

• Custom packaging options (100 mL to 500 L – bottles or bags)

• Small-scale batches for R&D or scale-up trials

• Free warehousing and reserved lots for consistency

🧬 Ideal for Transient Protein Expression in HEK293 Cells

Our media are specifically formulated for high-density growth and transient transfection efficiency with:

• PEI or lipid-based reagents

• Plasmid DNA

• mRNA expression vectors

Applications include:

• Recombinant antibodies

• Therapeutic enzymes

• Gene editing tools (CRISPR/Cas9 delivery)

• AAV and lentiviral production

🔬 You’ll achieve robust growth, improved yield, and minimal adaptation time – even for cells previously maintained in serum-containing systems.

🌍 Made in GMP-Compliant Facilities – With Global Reach

Currently, production takes place in a certified partner facility in China, operating under:

• GMP-compliant protocols

• ISO-certified cleanrooms

• Documentation suitable for EU and US regulatory needs

However, we are already planning a local EU production site (Germany), enabling faster turnaround and qualification for future GMP-certified processes.

🧪 Batch Sizes That Fit Your Needs

Whether you’re developing a platform process or scaling up for commercial production, flexibility matters. We offer:

You decide what your process needs – we take care of the logistics, documentation, and lot management.

🛡️ Traceability and Documentation You Can Trust

Every batch is shipped with:

• Certificate of Analysis (CoA)

• Full raw material traceability

• BSE/TSE-free statement

• Sterility and endotoxin testing

• Lot reservation (upon request)

• Transport temperature monitoring (if applicable)

Need additional quality checks for your QMS? We support pre-qualification with technical data packages.

💰 Competitive Pricing with Seamless Service

Despite being GMP-ready and fully traceable, our pricing remains substantially below comparable products from leading suppliers like Thermo Fisher, Lonza, or Cytiva.

How?

• No global brand premiums

• Lean production & logistics network

• Centralized EU warehousing

• Direct, personal consultation (no anonymous webshops)

We even support free sample requests and price matching in many cases.

📦 Request a sample or consultation

🔄 From Media to Monitoring – One Supplier, Multiple Solutions

In addition to culture media, we support your entire expression workflow with:

• Human Serum Albumin (recombinant or plasma-derived)
  🔗 rHSA for formulation

• Single-Use Bags for upstream/downstream
  🔗 Bioprocessing bags

• Live-Cell Imagers (zenCELL owl) for expression & health tracking
  🔗 zenCELL owl

• Human serum and plasma for custom supplementing
  🔗 Blood Products

🌟 Why Choose Seamless Bio?

✅ German-based company with international sourcing
✅ Transparent, fast delivery throughout EU, UK & CH
✅ No minimum order quantity
✅ Dedicated account support
✅ Free consultation for custom formulations
✅ Always available – never anonymous

📩 Let’s Build Better Media Together